STIMULATION OF EXTRACELLULAR PHYTASE PRODUCTION FROM BACILLUS SUBTILISBY POWDERED RICE BRAN IN SLURRY FERMENTATIONR. AGALYA DEVI, RAHUL KUMAR AND K. BALAKRISHNAN
Phytase production profile of Bacillus subtiliswas investigated here in submerged fermentation with and without enhanced level of fortification by powdered rice bran (Slurry fermentation). Incorporation of rice bran as substrate particles (5% w/v) in nutrient broth resulted in 5.11 times better yield of phytase when compared with un-supplemented broth. Among the different substrate particles introduced, rice bran was found to be inducing better phytase productivity. Substrate incorporation at varied concentrations (between 1% - 15% w/v) gave better phytase titers at 9% w/v (41.94 U/mL) of incorporation and 32 hrs of harvest. Acidic medium pH, addition of surfactants after sterilization and bioprocess carried out at 40°C were all found to have positive influence on the enzyme titers in the fermentation samples. Suitability of slurry fermentation for phytase production established. Higher enzyme activity at 40°C and acidic medium pH reiterate the suitability of this phytase as a feed premix for poultry.
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