Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 4, 2013; Page No.(647-654)

MICROBIOLOGICAL STUDY OF DRINKING WATER: QUALITATIVE AND QUANTITATIVE APPROACH

TASNIA AHMED, MRITYUNJOY ACHARJEE, MD. SHOHANUR RAHMAN, MONIRUNNESSA MEGHLA, JANIFAR JAMAL, SAURAB KISHORE MUNSHI AND RASHED NOOR

Abstract

Consumption of drinking water contaminated with fecally originated pathogenic bacteria is mostly responsible for the onset of water borne disease outbreaks especially in developing countries. Current study attempted to analyze 25 treated drinking water samples both qualitative- and quantitatively from different areas of Dhaka metropolis, Bangladesh where 90% cases of diseases (dysentery, typhoid, cholera and diarrhea) have long been reported due to the water borne microorganisms diseases. Through the most probable number (MPN) method, 5 samples out of 25 were found to be non-potable as they had been contaminated with Escherichia coliindicating the risk for fecal contamination responsible for disease outbreaks. Other contaminating Gram negative bacteria were characterized as Klebsiellaspp., Alcaligenes faecalis, Pseudomonasspp. and Aeromonasspp. Interestingly the presence of E. coliwas detected in the same 5 samples within a range of 3.0×102 to 1.7×10 5 cfu/ml by the conventional cultural and biochemical methods. Moreover, a huge array of other pathogenic bacteria was also detected (5.0×102 - 3.5×10 8 cfu/mL) through this method. Further detection of drug resistance traits among the bacterial isolates would be of public health significance.

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