Pollution Research Paper

Vol.31, Issue 1, 2012; Page No.(25-32)

ISOLATION AND CHARACTERIZATION OF PYRENE DEGRADING BACTERIA

ANUPAMA DEORA, RUPA GIRI, SUNITA SUNEJA*, SNEH GOYAL AND KAMLESH KUKREJA

Abstract

Contamination of polycyclic aromatic hydrocarbons (PAHs) which are toxic environmental pollutants causes public health risks and raises environmental concerns. One hundred and seven bacterial isolates were obtained from PAH (pyrene) contaminated soil samples by enrichment culture technique. Eight bacterial isolates showed zone of pyrene utilization around their colonies in the presence of glucose (0.2%) on L9 Minimal medium agar plates while one showed the utilization zone in the absence of glucose also. All the eight isolates produced zone on complete medium plates also. Pyrene utilization was also checked in L9 Minimal broth using all the isolates. Two bacterial isolates viz. KLR 1 and KRRG 1 which showed more protein content and viable count in L9 broth amended with pyrene (1mM) as compared to others were selected for different carbon and nitrogen source utilization pattern on L9 MM agar plates. Out of 16 carbon sources and 9 nitrogen sources tested, good growth & large zone of pyrene utilization was observed with eight carbon & five nitrogen sources in both the isolates. However in liquid medium, both the isolates showed maximum viable count and protein content with glucose as carbon source and ammonium sulphate as nitrogen source. These isolates were characterized using morphological, physiological and biochemical tests and both the isolates were identified as Paenibacillus larvae.

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