Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 3, 2013; Page No.(589-592)

ROLE OF CARBON SOURCE AND ZYMOGRAM STUDIES ON AMYLASE FROM BACILLUS SP

G. AKHILA, T.VISHWANATHA AND K.G.SIDDALINGESHWARA

Abstract

Amylases are among the most important enzymes used in modern biotechnology particularly in the process involving starch hydrolysis. Screening of amylase producers were screened by starch plate assay. The influence of the carbon source on alpha-amylase production by Bacillus sp was quantified in carbon-limited carbon cultures. The following carbon sources were investigated: (different chain lengths), glycerol, lactose, inositol, arabinose and maltose each at 1%. Bacillus sp did not show positive results maltose as the sole carbon source, but lactose (16 IU) and arbinose (13 IU) showed maximum amylase production. Sodium chloride also used individual source for the production (20 IU) at optimum 2% level and zymogram studies were also carried out. Partial purification were carried out by ammonium sulphate salt precipitations at 80% were carried out and it showed 111.12U/ mg specific activity.

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