Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 3, 2013; Page No.(479-482)

BIOACTIVE COMPOUNDS PRODUCED BY ENDOPHYTIC FUNGI ISOLATED FROM BEACH MORNING GLORY (IPOMOEA PES-CAPRAE LINN.)

V. CHAIPACKDEE, S. PORNPAKAKUL AND P. CHOKRATIN

Abstract

Endophytic fungi were isolated from leaves, stems, petioles, flowers and seeds of Ipomoea pescaprae Linn. collected from beaches of Thailand. All of selected endophytic fungi were assayed for antimicrobial activity against 5 microorganisms; B. subtilis, S. aureus, E. coli, P. aeruginosa and C. albicans. Crude extract of endophytic fungus isolate Ch109 exhibited highest antimicrobial activity against tested microorganisms. Based on light microscope identification and DNA sequencing of the internal transcribed spacer (ITS) region, the fungal isolate Ch109 was identified as Eurotium amstelodami. Isolation of bioactive substances from mycelia of E. amstelodami by column chromatography gave 2 compounds including tetrahydroauroglaucin and flavoglaucin. Minimum inhibitory concentration (MIC), antioxidant activity and anticancer activity of both compounds were assayed.

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