ECO-BEHAVIORAL ASSESSMENT OF ORGANOPHOSPHATES AND BIO-PESTICIDES: A COMPARATIVE CASE STUDY OF PESTICIDAL INFLUENCE ON THE GROWTH OF SELECTED BACTERIAL POPULATION (PSEUDOMONAS) IN SOIL BIOTAMukesh Nitin, Agatha. S. Khalko, Rajnish Kumar, Ruma Sinha, Nazia Sultan and Yogesh Kumar
A Gram-negative aerobic bacterium designated as Pseudomonas mendocina (PC19) was isolated from the soil collected from ICAR Research Complex, Eastern Region, Plandu, Ranchi, Jharkhand (India). Identification was done using 16S rDNA method. It plays important role in bioremediation and growth stimulation in plant. Laboratory assays were done to evaluate the effect of four different organophosphates (malathion, dimethoate, chlorpyrifos, phorate) and biopesticides (biosanjeevani, biosoft, neemta and leaves extract of species Karanj), applied at three different doses separately (RD,2RD,10RD) in soil sample. Study was carried for 40 days at 10 days regular interval. It was observed that maximum population mean observed for Karanj RD at 10 day (2.2 ± 0.62), 20 day (2.26 ± 0.40), 30 day (2.86 ± 0.32) and 40 day (2.86 ± 0.51), while minimum population mean was observed for malathion 10RD at 1 day (0.001 ± 0.001), 10 day (0), 20 day (0), 30 day (0.0003 ± 0.0005) and 40 day (0.0001 ± 0.0001). Statistical analysis was done using one way ANOVA resulted in comparative study between Organophosphates (OP) and Biopesticides (BP) influence on bacterial population with respect to control. OP and BP at RD, 2RD and 10RD concentration was justified by ANOVA resulted variance in bacterial colony growth (F value = 6.08, P value = <.0.0001, d.f. = 24).
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