Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 2, 2013; Page No.(353-358)

EVALUATION OF GENETIC DIVERSITY IN SOME BANANA HYBRIDS USING RAPD MARKERS

SUKHEN CHANDRA DAS AND T.N. BALAMOHAN

Abstract

Bananas (Musa spp.) are mostly diploid or triploid with various combinations of genomes inherited from their ancestors. Random amplified polymorphic DNA (RAPD) markers were used for evaluation of genetic diversity of some synthetic Banana Hybrids. A total of 13 random primers were evaluated for banana hybrids based on RAPD polymorphism. Diversity analysis carried out by using RAPD markers in some Banana hybrids revealed the confirmation of the hybridization along with the phylogenetic relationship of the hybrids with their parents. Out of the 13 primers studied, all the primers exhibited scorable markers. However, the maximum number of loci amplified was 18 with primer OPZ 12 and the maximum polymorphism percentage was obtained with the primer OPZ 16.

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