Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 2, 2013; Page No.(291-297)

EFFECT OF INDUCED STRESS IN SILKWORM AND ASSESSMENT OF MOLECULAR MECHANISM OF HEAT SHOCK PROTEIN

S. MEENASHI AND V. PRABAKARAN

Abstract

Bombyx mori L(Lepidoptera Bombycid) which feed on mulberry leaves are widely used for silk production. The Bombyx mori is one of the most thermal sensitive organisms. Heat shock proteins were presumed to ensure survival under stressful conditions by involvement in damage protection or damage repair due to their action as molecular chaperons. Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat, pressure and UV radiations. The effect of heat shock in the whole larvae on various physiological parameters and proteins of haemolymph were analyzed and it was found that the appearance of 90,73,65,44 and 37 kda protein were assessed. Among which 90 kda protein is prominently seen in the haemolymph samples. An analysis of gut epithelium of the normal Bombyx mori revealed the presence of microorganisms such as Bacillus, Lactobacillus and Staphylococcus sp where as the diseased gut epithelium revealed the presence of Salmonella, Serratia and Cornybacterium. The predominant fungal isolates such as Aspergillus niger, Penicillin and Aspergillus orizae were identified in the gut epithelium of silkworm. Further molecular characterization by 16SrRNA revealed the genes of the isolates showed 780 bp DNA fragments by PCR amplification.

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