Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 4, 2009; Page No.(903-906 )

IN-VITRO REGENERATION OF SIEGESBECKIA ORIENTALIS L., A MEDICINAL PLANT

R. GEETHA AND G.V. GOPAL

Abstract

Multiple shoots were induced from nodal explants of Siegesbeckia orientalis L. on Murashige and skoog's basal medium supplemented with 1mg/L Benzyl Amino Purine within 4 weeks of culture.These shoots were sub cultured for in vitro rooting with MS medium supplemented with different auxins at various concentrations and the best results were obtained on MS+1mg/L Indole Butyric Acid. In vitro flowering was observed on a medium supplemented with 2 mg/L Benzyl Amino Purine within six weeks of culture. The plantlets were hardened in a 2:1 potting mixture of sand and soil and were established under greenhouse conditions. Hence, in vitro propagation protocol of siegsbeckia orientalis has been standardized.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.150 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.