Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 4, 2009; Page No.(837-842 )

ACTIVITY GUIDED FRACTIONATION AND PHYTOCHEMICAL ANALYSIS OF BRUGLIIERA CYLINDRICA LEAF

B. L. JADHAV, S.M. MANE AND S.S. JAMKHEDKAR

Abstract

Antimicrobial properties and phytochemical analysis of Bruguiera cylindrica leaf were studied. The cold and hot extracts of leaf were made in petroleum-ether, chloroform, acetone, ethanol and methanol solvents and tested against broad spectrum human pathogens. The crude and activity guided ethanol fraction inhibited growth of most of the test organism. The minimum inhibitory concentration (MIC) ranged from 0.5mg/mL to 5mg/mL.The growth of S. typhi and B. subtilis was inhibited at 0.5mg/mL and for rest of the test organisms it was 5mg/mL. The activity guided fractionation indicated bioactive principles in only the crude ethanol and activity guided ethanol fraction (fraction `g'). The HPTLC analysis and various confirmatory tests for bioactive principles of the ethanolic fraction revealed the presence of anthraglycosides, cardiac glycosides, bitter principles, essential oils, valeportriates and coumarins in the ethanol fraction.

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