IN VITRO SHOOT BUD PROLIFERATION FROM AXILLARY BUDS OF SIMAROUBA (SIMAROLIBA GLAUCA L.)L. PRASANTHI, P. SUDHAKAR, SREELATHA, K. REKHA RANI, P. LATHA, I. RAJESWARI AND RAJA REDDY
Protocols for in vitro propagation of Sintaroulm gialloa through axillary bud proliferation and adventitious shoot bud regeneration from nodal segments have been established. Various factors such as browning, media type and plant growth regulators influence the in vitro propagation of S. glatica. These factors have successfully been addressed after addition of media additives, standardizing the media type and use of plant growth regulators at varying concentrations. Polyvinylpyrollidone (PVP 1%) was found effective for successful partial removal of phenolic compounds and obtaining maximum percent survival of explants. The maximum number of shoots (4-5/explant) was obtained in Murashige and Skoog's (MS) basal medium supplemented with BAP (1.5 mgL-1) and kinetin (1.0 mgL-1). Of all the media types tried, MS was found to be the best. Kinetin and BAP was found superior for explant establishment and multiplication.
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