Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 4, 2009; Page No.(757-760 )

A METHOD TO IMPROVE THE SENSITIVITY OF SEMEN CULTURE IN IDENTIFYING THE BACTERIOSPERMIA IN MALE INFERTILITY

M.R. SUSEELA AND D. JOSEPH PUSHPA

Abstract

The study was designed to determine the increase in the culture Positivity in semen culture by routine culture method and after centrifugation of the same semen samples. Methodology: Semen samples of symptomatic and asymptomatic infertile males were studied for bacteriospermia by conventional culture method and culture after centrifugation of the same samples. Culture plates were examined and colonies were identified. Age of the patients, sperm count and type of motility and morphology of spermatozoa were also recorded. Results: Bacteriospermia in semen culture showed the growth of E.coli, Staphylococcus Aureus, Enterococci, Ureaplastna urealyticum, Klebsiella, Pseudomonas, Mycoplasma, Gonococci, Providentia and Proteus. Organisms like E.coli, Enterococci and Coagulase positive Staphylococci play an important role in sperm impairment. Semen culture of asymptomatic infertile males showed increased culture positivity than the semen Culture of symptomatic infertile males. Semen culture after centrifugation improved the identification of bacterial Growth especially in asymptomatic young males of infertile group.

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