Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 4, 2009; Page No.(777-733 )

EXPRESSION OF STRESS RESPONSIVE GENES DUE TO ENDOSULFAN TOXICITY ON MODEL ORGANISM C 4ENO RHA BMUS ELEGANS

SURJO PRABHA PAUL SEEBA MANOJ, VASUDHA NAGRAJ, SUOHA DEVRAJ. AND M. RAMYA

Abstract

In order to identify genes that are differentially expressed as a consequence of oxidative stress on exposure to endosulfan in the current study mIZNA RI -PCR differential display method was adopted using 2x3 primer combinations following treatment with different concentrations of endosulfan on model organism Caenorhabditis elegans. On blasting the result the induced band was identified as HSP 70(HSP-1 and HSP-2) heat shock protein which showed homology to mouse and human sequences. Thus we demonstrate that C.elegans can be used as an model organism for evaluating stress response.

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