Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 3, 2009; Page No.(653-656 )

ISOLATION, CHARACTERIZATION AND EVALUVATION OF PSEUDOMONAS FLUORESCENS AGAINST SCLEROTIUM ROLFSII AS BIOCONTROL AGENT

C.N. MURUGA LA KSHMI, R. ANAND AND K. BHUVANESWARI

Abstract

The present investigation focuses on the screening of potential biocontrol agents against the stem rot pathogen of groundnut namely Sclerotium rolfsii. Among the two variety of antagonists screened namely P. f1uorescens, isolated from rhizosphere of groundnut and P fluorescens MTCC 1749, both species were very effective in controlling the growth of S.rolfsil both in vitro and in vivo conditions. Culture filtrate of Pseudomonas isolate and MTCC 1749 were more effective in inhibiting the mycelial growth, thereby causing reduction in dry mycelial weight of S.rolfsii. The outcome of this investigation was derived based on greenhouse experiments that confirm the antagonistic potential of the test biocontrol agents.

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