Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 3, 2009; Page No.(611-620 )

POLYETHYLENE GLYCOL-INDUCED FUSION OF YEAST AND FILAMENTOUS FUNGI PROTOPLASTS : ELECTRON MICROSCOPIC STUDY

NIVEDITA SINGH AND U. S. BAGDE

Abstract

The recent availability of ready-to-use and affordable DNA technologies has resulted in an upsurge of interest in mushroom strain improvement using the techniques of genetic recombination that produce viable intergeneric crosses with the aid of protoplast fusion. In view of this, an effort has been made to develop simple and sensitive methods for the protoplast fusion of two distantly related fungal species, a basidiomycetous filamentous fungi Volvariella diplasia, a popular edible mushroom, with ascomyceteous yeast Williopsis saturnus to produce a resultant fusant that exhibits the desirable traits of both parents in terms of enhanced values of vitamins of the B group thiamine, riboflavin and niacin and protein content of the edible mushroom. Since the goal was the production of a basidiomycetous filamentous fungi containing some of the desirable characteristics of an ascomycetous yeast, protoplast fusion technology could be used successfully to circumvent the barriers to conventional cell fusion that normally do not allow crosses between distantly related and unrelated strains and introduction of foreign genetic information into the fungal cell.

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