Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 3, 2009; Page No.(605-609 )

PROTOPLASTS OF YEAST WILLIOPSIS SATURNUS: FORMATION, REGENERATION AND FINE STRUCTURE

NIVEDITA SINGH AND U.S. BAGDE

Abstract

Spherical, osmotically sensitive protoplasts were efficiently isolated from young cells of yeast Williopsis saturnus by the 1:‘,/tic action of cell wall lysing enzyme Lyticase at a concentration of 0.25%. A variety of experimental conditions were investigated in order to achieve optimal conditions for viable protoplast formation and regeneration. Detailed analysis of different factors affecting the synthesis of protoplasts has been reported. The most suitable conditions for protoplasting have been optimized using 20 hour old culture and 0.8M sorbitol as the most suitable osmotic stabilizer. Protoplast formation was spectrophotometrically estimated to be over 98% complete in the end of 90 minutes. Transmission electron micrographs detailed the protoplast ultrastructure and showed the preparations to be completely devoid of cell wall.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 21, Issue 4, 2019
Vol 21, Nov. Suppl. Issue, 2019
Vol 21, Issue 3, 2019
Vol 21, Issue 2, 2019
Vol 21, Issue 1, 2019
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!