Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 2, 2009; Page No.(457-460 )

DETECTION OF MYCOBACTERIUM AVIUM SUB SP. PARATUBERCULOSIS EXCRETION IN GOAT MILK

B.S.M. RONALD, RITA NARAYANAN, N. KRISHNAKUMAR, S. BAEGAN AND B. MURALI MANOHAR

Abstract

The disputed link between the presence of Alticobacterium avium subsp. paratuberculosis (Map) in foods and the occurrence of Crohn's disease in humans is of continuing concern for food manufacturers. Thirty milk samples and thirty faecal samples were collected from adult goats in an organized farm with previous history of Paralnberculosis. The milk and fecal samples were decontaminated with 0.9% hexa decyl -pyridium chloride (H PC) and antibiotic solution (Vancomycin, Nalidixic acid and Amphotericin B) and cultured in Herrold's egg yolk medium (HEYM) supplemented with Mycobactin J (Allied Monitors, USA). The results revealed that 43% of the goats were positive for Johne's by fecal culture and 13% were positive by milk culture. The Map organism formed smooth moist glistening colonies in HEYM after 3-4 months of incubation and were mycobactin dependent. Further Polyrnerase Chain Reaction was done for the samples targeting insertion sequence 900 (1S900). The milk and fecal samples revealed positivity in 27% and 60% samples respectively. This study revealed that Map is being excreted in milk, which could play an important role in dissemination of the disease to kids, which are highly susceptible. Similarly milk products from goat milk can also play a role in transmission of the disease in human beings..

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