Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 2, 2009; Page No.(415-417 )

IN VITRO EVALUATION OF ANTIBACTERIAL SPECTRUM OF FLOWER EXTRACTS OF HIBISCUS ROSA - SINENSIS

SARDAR APARNA AND B. S. SURWASE

Abstract

Hibiscus rosa-sinensis L. is a shrub and grown as an ornamental plant. It is used against various ailments like cough, fever, cystitis, gonorrhea, bronchial catarrh, boils, asthma, headache, menstrual irregularities, prostate disorders, diarrhea etc. It also acts as antispasmodic for uterine and intestinal spasms. The flowers are considered as emollient and demulcent. Leaves are anodyne, emollient and aperients. Ethanol, methanol and distilled water hot extracts of flowers were investigated for their antimicrobial activities. The growth inhibition was determined using well plate method against gram positive and gram negative bacteria. In over all, among the tested solvent extracts, ethanolic extract was most active against most of the selected bacteria than methanolic and distilled water extract. E. coli CSH 57 and C. glutamicion are more resistant, where as all other selected bacteria are sensitive to ethanolic extract. B. subtilis was more sensitive to methanolic extract, where as E. coli CSH 57 and B. megaterium were sensitive to water extract. Over all, standard antibiotics showed more zone of inhibition than various solvent extracts.

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