Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 2, 2009; Page No.(269-273 )

RED GRAM HUSK - AS A NOVEL INEXPENSIVE SUBSTRATE FOR THE PRODUCTION OF ALKALINE PROTEASE USING BACILLUS SUB TILTS SVR - 07

M. NARSI REDDY, K. SWATHI, B.NAGAMANI, S. VENKATESHWAR, L. VENKATESHWAR RAO AND CH. SUBBA RAO

Abstract

Alkaline protease production under solid-state fermentation was investigated using isolated alkalophilic Bacillus subtilis SVR-07. Among all agro -industrial waste material evaluated, redgram husk supported maximum protease production. Solid material particle size regulated the enzyme production and yield was improved with the supplementation of carbon and nitrogen sources to the solid medium. Maximumenzyme production was achieved with 1.5% maltose and 2.0% yeast extract with 371% increase than control. Glucose did not repress enzyme production but inorganic nitrogen sources showed little negative impact. The physiological fermentation factors such as pH of the medium (pH 9.0) moisture content (150%), incubation time (48 h) and inoculum level (3%) played a vital role in alkaline protease production. The enzyme production was found to be associated with the growth of the bacterial culture.

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