Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 11, Issue 1, 2009; Page No.(185-188 )

OPTIMIZING THE CONDITIONS OF L-GLUTAMIC ACID RODUCTION BY CORYN E BACTERIUM GLUTAMICUM

ARUN SASI, A. KALIDOSS, M. RAVIKUMAR, A. CHANTHRU, R. BHAKYARAJ AND N. YOGANANTH

Abstract

Amino acid such as glutamic acid were synthesized has a unique physical and chemical properties. It have many application is good, medical and pharmaceutical field. Corynebacterium glutamicum was produced, large amount of L-glutamic acid than other organisms. Culture collected from MTCC institute of microbial technology. To optimize the production of L-Glutamic acid by changing the carbon source like dextrose, lactose, glucose, lactose, mannitolcl, mannose, starch and sucrose. Temperature 30°C, 37°C and pH were adjusted to 5.5, 6.5, 7.0, 7.5 respectively. Biotin were used at a concentration of 200 pL, 100 pL. Whey will act as an additional and cheaper carbon source. When biotin of 100pg/mL is liked, L-glutamic was produced at maximum of 300 pg/mL with lactose act as carbon source and temperature of 37°C for production. When biotin of 100 pL was added to whey the production was maximum of 275 pg/mL with temperature of 37°C and pH of 6.5.

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