BIOASSAY DIRECTED SCREENING OF SELECTED MEDICINAL PLANTS FOR ANTIBACTERIAL PROTEIN ACTIVITYS.F. MALEEKA BEGUM AND V. VIMAL NATHAbstract Medicinal plants hold good promises as they are proved be the best antibacterial drugs and more over the active component posses the 'potentiality of broad spectrum antimicrobial property. Crude extracts from the leaves of Plants viz.,Lippia nodiflora Aristolochia bracteolata, Calophyllum ionophyllum and Anacardium species possess considerable antibacterial activity against 3 bacterial pathogens viz, Bacillus substilis, E.coli Pseudomonas acroginosa. The solvents used for the extraction includes chloroform, petroleum ether and ethyl acetate. All the solvent extracts elaborated inhibitory effect, however with varying degree of magnitude. On a overall view, all the test extracts showed moderate to high bactericidal activity of the following order of magnitude : Aristolochia. bracteolata > Anacardium sp > Calophyllum ionophyllum > Lippia nod iflora. The active component responsible for the antibacterial activity were collected by TLC chromatograph. The chromatogram obtained was dissolved in methnolic solution and antibacterial activity was carried out and it was inferred that the eluted compounds have greater potential antibacterial property than the solvent extracts. Moreover, Rf value coincided well with the standard antibiotics like Gentamycin, Streptomycin and chloramphenicol.
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