STANDARDIZATION OF GROWTH MEDIUM AND INCUBATION PERIOD FOR DETECTION AND QUANTIFICATION OF AFLATOXIN PRODUCTION BY TLC METHODP. SUDHAKAR, Y. SREENIVASULU AND P. LATHA
In the present investigation, best culture medium for higher mycelial growth based on the dry weight of the fungal biomass, optimum incubation time for higher aflatoxin production and standardization of amount of aflatoxin to be spotted on TLC plate for better illumination were studied. The results revealed that mycelial mat weight and total toxin production were highest on SMKY liquid medium compared to PDA, AFPA and YES media. Among the different concentrations of aflatoxin samples viz., 2, 5, 10,20 and 30pL plated on silica plate, 20pL of sample and at 7 days of incubation period showed bright blue illumination in UV transilluminator.
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