Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 4, 2008; Page No.(879-882 )

STUDY ON THE EFFICIENCY OF CAPRINE MACROPHAGES AND B CELLS IN THE GENERATION OF CYTOTOXIC T LYMPHOCYTES

M. ANANDA CHITRA AND G. C. RAM

Abstract

Cytotoxic T lymphocytes activity in culture containing caprine macrophages(M) and B cells were assessed in this study. Antigen pulsed autologous macrophages and B cells were used as target cells and cytotoxicity was assessed by MTT colorimetric assay, fluorescentdyemix and DNA quantitation assays for apoptosis. M. paratuberculosis pulsed WI) and B cells were subjected to higher cytotoxicity than S.aureus or tetanus toxoid (TT) pulsed with WI) and B cells. Cytolytic activity against S.aureus pulsed Mq.)was greater than S. aureus pulsed B cells. Macrophages showed higher cytotoxicity in the antigen pulsed culture than B cells culture. Cytotoxicity against TT primed culture was lesser than against particulate antigen primed culture. This may be due to the effect of alum adjuvant used to sensitize the goat with IT.

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