MECHANISM OF DRUG RESISTANCE IN STAPHYLOCOCCUS A LIRELISNILIMA LANKESHWAR AND U. S. BAGDEAbstract The emergence of drug resistant bacteria is posing a major problem in antimicrobial therapy. The frequency varies with the organism and the drug used. If treatment with drug kills 99% of the bacteria, the remaining 1% will breed a new generation of germs that will not be affected by the particular drug. They will probably pass that resistance on to bacteria of other species. A possible mechanism of resistance to Ciprofloxacin and Sparfloxacin in Staphylococcus aureus. was studied. The growth of sensitive Staphylococcus aureus was completely inhibited at 0.5pg/ml, of Ciprofloxacin and lpg/mL concentration of Sparfloxacin, while the resistant strain tolerated even 5 pg/rnI, concentration of Ciprofloxacin and 50 pg/mL concentration of Spa rfloxacin. Mechanism of resistance was found to be the presence of plasmid. Plasrnid was analyzed by agarose gel electrophoresis. Plasrnid curing was successful with 10` sodium dodecyl sulfate (SDS) treatment. All colonies on plate after SUS treatment were tested for drug resistance on nutrient agar containing diffrent concentrations of drugs,
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