Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 4, 2008; Page No.(797-800 )

PHAGOCYTIC ACTIVATION OF A LUMINOL- DEPENDENT CHEMILUMINESCENCE IN CAPRINE MACROPHAGES AND B CELLS

M. ANANDA CHITRA AND G. C.RAM

Abstract

The quantitative luminol-dependent chemiluminescence (LDCL) assay was used to assess the endocytic capacity of caprine MO and B cells, using various stimuli. MO was potent producer of oxidative burst to all stimuli and B cells had weak endocytic capacity. Zymosan induced greater LDCL response followed by M.paratuberculosis, S.aureus and SRBC. Antigen presenting cells generated LDCL response was greater to live organisms than killed organisms. M. paratuberculosis induced LDCL more potently than S.aureus. Colchicine caused a statistically significant (p<0.05) inhibition of endocytosis but it did not completely abrogate the endocytic process.

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