THE EFFECTS OF TEMPERATURE, PH AND CARBON SOURCES ON ANTIMICROBIAL PEPTIDE AN5-1 PRODUCTION USING PAENIBACILLUS ALVEI AN 5BASSAM ALKOTAINI, NURINA ANUAR AND ABDUL AMIR HASSAN KADHUM
Paenibacillus alveiAN5 has been recently isolated from Palm Oil Mill Sludge with the ability to produce a secreted antimicrobial peptide AN5-1. This study indicated the effect of growth factors including incubating temperature, initial pH and different carbon sources on both P. alvei AN5 growth and AN5-1 production. It was noted that, the highest produced amount of AN5-1 were obtained when optimum growth occurred. P.alvei AN5 was grown in Modified Landy Medium at different temperatures; the highest amount produced of AN5-1 was 80 AU/mL at 30°C. In addition, the production was obviously influenced within the pH range of 4-9. At the extreme initial pH values of 4, 5 and 10, the production was depressed. The highest amount produced of AN5-1 (80 AU/mL) was obtained at initial pH of 7. The production of AN5-1 was strongly influenced by the variation of carbon sources, where it was obviously enhanced using glucose; meanwhile, starch and glycerol reduced the production of AN5- 1. The minimum glucose concentrations required to achieve the highest amount produced of AN5-1 was 20 g/L. The more P. alvei AN5 grows, the more antimicrobial peptide AN5-1 produces.
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