Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15 Issue 1, 2013; Page No.(159-164)

EXPRESSION OF TLR AND - ACTIN IN SHRIMP (PENAEUS MONODON) TISSUES INDUCED BY IN VIVO INFECTION WITH VIBRIO SPECIES

P. DHASARATHAN, S. PAULSI AND A.H.M. HARISH

Abstract

Shrimp suffers high morbidity and mortality in infection, presumably because of the lack of a fully adaptive and innate immune system. Interest in understanding shrimp immunity has increased because of its importance in disease control. In this study the expression of Toll like receptor (TLR) and ß- actin genes were isolated and sequenced from the tiger shrimp, P. monodon after the experimental infection of Vibrio species. Constitutive expression of the TLR and ß- actin genes were detected by Reverse transcription polymerase chain reaction (RT-PCR) in all shrimp organs including gut, hepatopancreas, gills and muscle. Comparative expression of mRNA of TLR in various organs of P.monodon showed that there was a good expression in gills compared to other organs. Identification of TLR and ß-actin will help to elucidate the toll pathway in shrimp innate immunity. PCR amplification of cDNA extracted from ß-actin in shrimp has an expected product size of 450bp. PCR amplification of cDNA with TLR specific primers has the product size of 800bp. Comparison of the expression of TLR in healthy and experimentally infected shrimp showed that the expression of TLR is more in experimentally infected shrimp. The PCR amplified sequence ß-actin and TLR sequence of shrimp were analyzed using the BLAST program. These finding suggest that TLR is an important factor in the shrimp (P.monodon) innate immune response against bacterial infection.

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