EVALUATION OF A NOVEL IGG-ELISA FOR SERODIAGNOSIS OF LEPTOSPIROSIS USING RECOMBINANT LIPL32 ANTIGEN OF L. INTERROGANS SEROVAR ICTEROHAEMORRHAGIAE IN DOGSM. SUBATFIRA, T. M. A. SENTHILKUMAR, G. L. RAMYA AND P. RAMADASS
A simple IgG ELISA was developed to overcome some of the limitations of conventional microscopic agglutination test in the diagnosis of canine leptospirosis. Recombinanat protein based IgG ELISA is a suitable and safe procedure for screening of large number of sera which lacks non-specific moieties present in whole cell preparations. Among the total of 533 canine sera tested, the sensitivity and specificity of IgG ELISA relative to MAT were 92.38% and 81.6%. Results indicated that the assay can be easily performed, and avoids the risk of infection in laboratory workers, and it seems to be a practical and suitable tool for serodiagnosis of leptospirosis.
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