Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 2, 2008; Page No.(425-427)

EVALUATION OF USING ENZYME-LINKED IMMONOSORBENT ASSAY (ELISA) FOR THE DETERMINATION OF PAHS IN WETLAND AND RIVER WATERS

P.S. TALEBI, S.M. TALEBI A. ABDOLAHHI AND S.V. HOSSEIN

Abstract

The possibility of determination of PA Hs in river and wetland waters using enzym-linked immonosorbent assay (ELISA) was investigated. Several water samples were collected from river Zayandeh-Rud and Ghav-Khooni wetland and analyzed by both ELISA and GC-MS methods and the results showed that there is a considerable differences between the PAHs concentrations obtained by the two techniques. The difference between the results obtained by the two methods for wetland water was much less than those obtained for river water. Further investigation and analysis of individual PAHs in the samples showed that the concentrations of heavier PAHs that ELISA is more sensitive to them are higher in wetland water. However, ELISA is a rapid and less expensive method that can be used for the roughly evaluation of PAHs pollution in water bodies.

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