EXTRACTION AND CHARACTERIZATION OF BIOACTIVE SUBSTANCES FROM RAY FISHES AND THEIR ANTIBACTERIAL PROPERTYV. RAVITCHANDIRANE AND A.YOGAMOORTHIAbstract The ray fishes Viz. Dasyatis zygei and Narcine brunnae, collected from Puducherry (Pundicherry) coastal waters were screened for their antibacterial property against five common bacterial pathogens. The ether extract of both fishes showed very significant inhibitory effect against Vibrio cholerae in comparison with known antibiotic-Tetracycline. The biochemical elucidation of crude ether extracts showed the presence of salicylic acid and sulphonic acid which are presumed to be the active substance for such antibacterial activity. The biochemical characterization of crude petroleum ether extract of Narcine brunnae showed no such bioactive substance/activity as exhibited by ether extracts of Dasyatis zygei and Narcine brunnae.
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