Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 2, 2008; Page No.(229-234)

MOLECULAR CHARACTERIZATION OF PLASMA PROTEINS TO CONFIRM THE RISK FACTOR FOR ROGRESSION TO MULTIPLE MYELOMA

G. RENUGA, A. BABU AND K. R. ARUMUGAM

Abstract

The diagnosis of multiple mveloma is often made incidentally during routine blood tests for other conditions. A diagnosis of multiple myeloma is difficult to make on the basis of any single laboratory test result. The biochemical investigation of mu ltiple myeloma and its molecular characterization based on agarose gel electrophoresis and bone marrow aspirates were examined from human samples.Experimental samples showed increased serum calcium, total protein level and decreased serum albumin level. Bence Jones protein precipitate was present in all experimental samples and were showed the narrow and intense band of 'M protein on gel. When compared all experimental samples no3 could be considered a severely affected patient, due to the increased level of serum calcium, total protein and decreased level of serum albumin than other experimental cases. Bone marrow aspirate of sample no3 clearly showed the differentiation of normal plasma cells to myeloma plasma cells. The biochemical and molecular techniques used for this project has been considered as an early diagnostic tool for cancerous suspected cases. Any physician can apply this molecular characterization methodology to identity multiple myeloma condition at earlier among cancerous cases.

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