LYMPHOCYTES TRANSFORMATION AGAINST THE ANTIGEN OBTAINED FROM PATHOGENS SUSPECTED FOR EUSALOK TRIPATM AND T.A. QURESHI
The lymphocytes are obtained by the culture of head kidney of a pre-exposed Channa gachita with three suspected pathogens. For this purpose three antigens are selected from three different suspected pathogens i.e. SHRV (RV-19E), Aeroinonas hydrophila (RS-III) and Aphanomyces invadans (AH-III). The net cpm is observed against the C-Protein of SHRV and it showed a dose dependent raise. The maximum cpm is observed at 100 g concentration while lowest in case of 25 jig concentration but the maximum count in this case is recorded after 144 hrs of exposure. In case of the antigen obtained from Aeromonas hydrophila the dose dependent cpm is observed and it was the maximum at 100 ug concentration while lowest at 25p.g concentration. Similarly in case of Aphanomyces invadans a dose dependent change is noticed. On comparing the above results, the most severe response was due to G-Protein from the SHRV, while least in case of LPS obtained from Aeromonas hydrophila.
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