Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 7 Issue 4, 2005; Page No.(791 -792)

MORPHOGENETIC RESPONSES OF PLUMULE, RADICLE AND COTYLEDON EXPLANTS OF SORGHUM VULGARE, PERS. IN VITRO

R.K.AHER AND L.N. NAIR

Abstract

Sorghum vulgare, Pers. is an agronomically important crop and used as food and fodder purpose. An in vitro multiplication system was developed to effectively propogate disease free variety of sorghum as well as analysis of biochemical alterations in vitro. In vitro culture was done by germinating the seeds aseptically and the cotyledon, plumule and radicle were used as explant on Murashige and Skoog's (MS) medium. The M.S. medium supplemented with different concentrations of growth regulators like 2, 4- D, Kinetin, BAP and LAA. Out of different explants and combinations callus developed well from radicle explants. Radicle explants proliferated well in M.S.+ 2, 4 - D (1 mg/1) + BAP (1mg/1). Callus used for organogenesis and detection of biochemical alterations. The in vitro raised plants were hardened in a green house and transplanted to the field successfully.

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