Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 6 Issue 4, 2004; Page No.(735-740 )

SCREENING OF OIL SEED CAKES FOR ISOLATION OF LIPOLYTIC FUNGI AND PARAMETRIC OPTIMIZATION OF LIPASE PRODUCTION BY THE SELECTED ISOLATE RHIZOPLIS SP. BTS-24

K.V.V.S.N. BAPIRAJU, P. SUJATHA, P. ELLAIH AND T. RAMANA

Abstract

Enriched samples from different oil seed cakes were screened for the isolation of lipolytic fungi by tributyrin agar clearing method and subsequently by cultivating the selected isolates under submerged fermentation conditions and assaying for their extracellular lipase producing capabilities. The promising isolate BTS-24 was identified as Rhizopus sp. and designated as Rhizopus sp. BTS-24. Nutritional and physical parameters influencing the lipase production by the selected isolate were investigated. The effect of different oils and oil seed cakes as sole carbon sources on the production of lipase indicated that gingelly oil cake was an efficient carbon source. The optimal lipase production was observed under the following conditions-initial pH of 5.0, incubation time of 72 hours, incubation temperature of 28`C, volume of the medium to volume of the flask ratio of 1:5 and agitation speed of 100 rpm.

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