Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 6 Issue 4, 2004; Page No.(675-679 )

BIOCHEMICAL ACTION OF BMNPV INFECTION ON CERTAIN TISSUES OF SILKWORM BOMBYX MORI L.

G. SINGARAVELU, K. DEEPA, P. PRABU AND M. SAKILA

Abstract

The incidence of viral borne diseases were more frequent as well as drastic in sericulture industry. In order to establish a thorough knowledge for a successful control of disease transmission it is imperative to understand the metabolic alterations in the infected silkworm Bombyx mori L. On the basis the present investigation has been undertaken. During the first day of V instar, Bombyx mori Nuclear polyhedrosis virus (BmNPV) was given through leaves to the experimental group in a concentration of 10 mg/larva. For the subsequent days of the V instar the larvae were fed with normal leaves of the MR2 variety. The impact of inoculation of BmNPV on silkworm larvae was carried out on 20 hours intervals. During every 20 hours intervals quantitative changes in the levels of important biomolecules such as glycogen, protein and reducing sugar was analysed on 3 target tissues namely the fatbody, midgut and haemolymph of Silkworm Bombyx mori L. Changes in the levels of this biomolecules envisage the metabolic stress that the silkworm Bombyx mori experiences due to NPV infection. The results were statistically analysed and discussed with pertinent literature.

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