VARIATIONS IN BACTERIAL BIOFILMS WITH REFERENCE TO NUTRIENT LEVELSB. PRADEEP, P.K. PANDEY AND S. AYYAPPANAbstract Biofilm formation depends on biotic and abiotic factors of which nutrient availability is one of the most important abiotic factor. In present investigation an attempt has been made to assess the bacterial flora associated with water and biofilms in aquaria involving treatments with and without fish. Additional nutrients released into the aquaria in the form of fish excreta quantified as 0.0107 ± 0.005 g/ fish/ day using a fish excreta collection unit. Five fingerlings of Calla calla (1.826±0.02g) were stocked in 40 litre aquaria having biofilteration system. A significant difference was observed between treatments in case of heterotrophic bacterial population associated with biofilm. This suggests that biofilm formation depends on nutrient availability as nutrients released in the form of fish excreta increased the rate of biofilm formation. Bacillus was the main bacterial genera associate with biofilrns followed by Staphylococcus, Pseudomonas, Streptococcus and Micrococcus.
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