Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 6 Issue 4, 2004; Page No.(607-608 )

OPTIMIZATION OF STORAGE TEMPERATURE FOR CAC12 COMPETENT CELLS FOR ROUTINE TRANSFORMATION OF ESCHERICHIA COLI WITH PLASMID DNA

DINESH YADAV AND N.K. SINGH

Abstract

Storage conditions for competent cells for carrying out routine transformation of E. coli using CaC12 method with plasmids of different size were optimized. Competent cells prepared by CaC12 method consistently gave transfr -mation efficiency of 2.8-2.4 x 106 transformants/pg of plasmid DNA when kept at 4°C in freeze upto 3 weeks. The efficiency of transformation was more or less same for plasmic-is of different sizes. Competent cells stored at —20°C was not very effective for long duration.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.