Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 6 Issue 3, 2004; Page No.(395-401 )

PRODUCTION AND PROPERTIES OF CELLULOLY TIC ENZYMES BY TRICHODERMA VIRIDE AND TRICHODERMA HARZIANLIM IN SUBMERGED FERMENTATION

M. VIJAY ANTONY S. MOHAN, P. KASINATHAN1 AND N. THAJUDDIN

Abstract

A complete set of cellulase comprising of endo glucanase, exo glucanase, 13-g1ucosidase and xylanase was produced by Trichoderma viride and Trichoderma harzianum by submerged fermentation. Enzymes production was maximum in 1% Avicel as carbon source and a combination of 0.6% yeast extract as nitrogen source at pH 5.0 on 5th day of incubation. Cellulase and xylanase of both the Spp. were optimally active at pH 4.8-5.2 and at 50°C. Stability of these enzymes was more than 60% at pH 4.0 -6.5 and at 45°C - 55°C.

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