BIODEGRADATION OF 2-, 3- AND 4- CHLOROPHENOLS BY A DEFINED MIXED CULTUREJ.B. CHAWLA AND A.O. INGLEAbstract Two bacterial strains, Acinetobacter calcoaceticus A-2 and Alcaligenes denitrificans J-3 were isolated from soil by enrichment culture technique. Under aerobic conditions, strain A-2 metabolized 3-chlorophenol (3-CP) and 4-chlorophenol (4-CP) effectively, while 2-chlorophenol (2-CP) was metabolized very slowly; each at 1001.iM initial concentration. The maximum degradable concentration of 2-CP, 3-CP and 4-CP with strain A-2 was 250 piM, 800 1.1M and 900 p_tvi respectively. Strain J-3 rapidly metabolized 2-CF with maximal degradable concentration of 2.3 mM; but could not metabolize 3-CP and 4-CP. The inhibition of 3-CF and 4-CF degradation in presence of 2-CP, with strain A-2, was seen but inhibition of 2-CF degradation in presence of 3-CP and 4-CP, with strain J-3, was not seen. The mixed culture of strain A-2 and strain J-3 brought about effective metabolism of all the three monochlorophenols in a mixture, while neither strain A2 nor strain J-3 could do so under similar conditions.
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