Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 6 Issue 1, 2004; Page No.(25-30 )

VARIATIONS IN PROTEIN BANDS AND ISOZYME DIVERSITY IN GRAIN AMARANTHS

K. HAZRA, N. MANDAL AND P.K. DAS

Abstract

Sixteen genotypes of Antarantlius Ilypoehondriacus, two of A. cruentus and A. caudatus were studied for seed protein variation, peroxidase and esterase isozyme diversity. The seed protein through SDS-PAGE gel recorded conspicuous banding pattern where emeritus genotypes maintained lesser number of bands than eaudatus and hypochondriacus. The latter two maintained more or less a close similarity in storage protein banding pattern Singnificantly only high molecular weight protein bands generated a high order of variation causing a distinct deviation among them. As regards peroxidase and esterase, emeritus displayed reduced number of isozyme bands. Considering both peroxidase and esterase to gether with seed storage protein cruentus remain distibctly separate from other two. The dendrogrammes constructed either from peroxidase and esterase or from seed protein confirmed the distinctness of cruentus that remained a class apart while caudatus and hypochondriacus overlapped suggesting a high order of phylogenetical diveragence of emeritus.

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