BIOCHEMICAL CHANGES IN SORGHUM DUE TO SPHACELOTHECA SORGHIR.K. AHERland L.N. NAIRAbstract Biochemical changes brought about by grain smut(Sphacelotheca sorghi) (Links) Clinton were analysed in both healthy and infected inflorescence of Sorghum. These included reducing sugar, proteins, nucleic acids, phenols, polyphenol oxidase and peroxidase were quantitatively detected. The amount of reducing sugar, phenols, proteins, RNA, DNA, polyphenol oxidase and peroxidase activity was detected using standard methods. The amount of reducing sugar decreased in infected inflorescence where as phenols, proteins and nucleic acids increased in infected inflorescence. Similarly there was increased polyphenol oxidase and peroxidase activity in infected inflorescence compared to the healthy.
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