Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 5 Issue 3, 2003; Page No.(345-348 )

SPATIAL AND TEMPORAL DIVERSITY IN PETROLEUM DEGRADING BACTERIAL POPULATION

ALAKNANDA C. PATEL AND S.K. GHOSH

Abstract

An attempt has been made to isolate and identify different types of petroleum biodegradable bacteria from petroleum spillage soils and crude oil samples obtained from two 0.N.G.C. project areas situated at different districts of Gujarat state-Ahmedabad and Mehsana. A total of 338 strains were isolated from twenty-eight samples (14 soils and 14 crude oil) with 50% of the samples collected from each district. The maximum and minimum numbers of isolates were from crude oil were 19 and 10 respectively. The number of isolates per soil sample was less compared to per crude oil sample and the ranges were 5 to 15 and 7 to 16 from Ahmedabad and Mehsana respectively. Only 14% (47) strains were potential hydrocarbon utilizers and 86% were secondary colonizer. All the potential degraders belonged to the genus Pseudomonas. Temporal and spatial variation of bacterial flora was studied.

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