Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 5 Issue 3, 2003; Page No.(297-300 )

OPTIMIZATION OF THE PROCESS CONDITIONS FOR CYCLODEXTRIN GLUCANOTRANSFERASE PRODUCTION USING RESPONSE SURFACE METHODOLOGY

HANAN MOAWIA IBRAHIM, WAN MOITTAR WAN YUSOFF, AIDIL ABDUL HAMID1, ROSLI MD ILLIAS AND OTHMAN OMAR

Abstract

Statistical experimental design was used to optimize the physical conditions such as temperature and speed of agitation in shake flask culture to enhance cyclodextrin glucanotransferase (CGTase) production from Bacillus sp. G1. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular CGTase was achieved (41.4 U/ml) when the speed of agitation and temperature were 147.7 rpm, 34.48°C respectively.

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