EFFECT OF AN ORGANOPHOSPHATE PESTICIDE IN THE INDUCED OVULATION OF MATURING AIR BREATHING FISH ANABAS TESTUDINEUS (BE) WITH HYPOTHALAMIC GONADOTROPIN RELEASING HORMONE FRACTION FROM CHANNA PUNCTATUS (B1.)C. GHOSH, A.K. RAY, S. BHATTACHARYA AND SHELLEY BHATTACHARYAAbstract Hypothalamic gonadotropin releasing hormone (GnRH) fraction, Acetone fraction II (AcF-II) was tested for inducing ovulation in an air-breathing fish, Anabas testudineus. The min'imal effective standard dosage of 37.5 IA e/ 100 g body weight was administered to the female fish in two instalments. An additional dose of 25l_a.,Y/100 g body weight of eGnRH to the female fish was required for complete maturation and ovulation. The male fish on the other hand, received single injection of c-GnRH (25 j.i.g1100 g body weight) at the time of second injection to the female. The experiment was carried out at the ambient temperature of 28°C and pH 7.4. The response of the female fish to metacid-50, an organophosophate pesticide, was also investigated in a similar condition with the same dosage of cGnR I I fraction (AcF-11). It was observed that the exposure of the fish to .safe concentration (0.106 ppb) of metacid50 for seven days resulted in complete failure in inducing maturation and ovulation, however with a similar dosage of cGnRH and which successful ovulation occurred in the control fish. This indicates that apparent safe concentration of metacid-50 in ambient water is sufficiently effective in producing reproductive inefficiency.
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