Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

STUDY ON ANTIBACTERIAL ACTIVITY OF TURMERIC

NAYANA NIKUMBH, SWAPNIL SHEVATE AND R.K. PATHAK

Abstract

In order to determine the antibacterial activity of turmeric, gram positive bacteria Staphylococcus aureus, Bacillus subtillis and gram negative bacteria E. coli, Pseudomonas fluorescence were collected .Water and solvent extract of turmeric were prepared by using hydro distillation method and solvent extraction method respectively. To detect potential antibacterial activity of turmeric, disc diffusion method was used. Molten Muller Hinton agar along with 0.2 mL of inoculum was poured into petri plates. The test compound was placed on the upper layer of the seeded agar plates. The plates were incubated at 37deg. C for 24 hrs. The inhibition activity of turmeric determined by measuring zone of absence of microbial growth. By using IR spectroscopy the functional compound Curcumin was detected. After quantitative analysis of curcumin it was found that 0.6506 g of curcumin is present per 100 gram of sample.