Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

DETERMINATION OF BLOOD ETHANOL IN OREOCHROMIS MOSSAMBICUS (PETERS): STUDIES USING GAS CHROMATOGRAPHY

SMITHA V. BHANU AND BABU PHILIP

Abstract

The objective of the present investigation was to study the effect of ethanol in the blood samples of O.mossambicus using external calibration method. The method involves direct injection of the blood sample under splitless mode into the gas chromatograph, without any pretreatment. Any sort of contamination of the gas chromatographic column was prevented by using glass liner in the injector. This liner, which acted as a precolumn, was partly filled with small glass beads. Injection was performed in between the glass beads. This injection technique between glass beads allows direct injection of the sample volumes without disturbing the gas chromatographic separation. GC-Varian CP 3800 gas chromatograph (GC-Varian CP 3800), equipped with a Flame ionization detector was employed for this. This method is considered to be rapid, accurate and sensitive.