BIOCHEMICAL QUANTIFICATION OF PROTEIN AND ITS RELATED ENZYME IN SEEDS AND SEEDLINGS OF LUFFA CYLINDRICA INFECTED WITH COLLETOTRICHUM ORBICULARENUPUR SADDA AND RASHMI VARMA
Biochemical estimation was carried out in seeds and seedlings of Luffa cylindrica infected with Colletotrichum orbiculare causing anthracnose disease. Colletotrichum orbicular infection was symptomatic carrying pin head like acervuli. The disease is characterised by water soaked lesions or yellowish areas which becomes dry and tear away. Quantification of proteins and its related enzyme was done in different plant parts of healthy and diseased counter parts. Different plant parts showed variation in their protein contents. Protein content was higher in case of anthracnose infected plant parts 1.54±, 0.58±, 0.31± and 0.26± mg/g in seed, stem, leaf and fruit respectively while in its healthy counterparts it was 0.84±, 0.32±, 0.27± and 0.18± mg/g in seed, stem, leaf and fruit respectively. Protease activity was higher in case of normal plant parts 0.021±, 0.015±, 0.029± and 0.024± units/sec/mg wt of fresh tissue of seed, stem, leaf and fruit than its diseased counterparts.
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