Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.13, Issue 3, 2011; Page No.(519-523)

COMPARISON OF THE PROTIEN AND DNA ACTIVITY IN THE LIVER AND SPLEEN OF ONE WEEK AND TWO WEEKS OLD BROILERS DURING CHRONIC AFLOTOXICOSIS

P.J.R. NATHANAEL AND V. VIVEKA VARDHANI

Abstract

The aflotoxins profoundly buffeted the immunological stamina of poultry birds and even causes mortality and various acute and chronic aliments. The present investigation is focused on the comparison of the protein and DNA response in the liver and spleen of 1 week and 2 weeks old broilers suffering with aflotoxicosis. Broilers of one week old (Group A;B) received 0.01ng/bird and 0.1ng/bird respectively; where as group C (untreated birds) kept for comparison. Broilers of two weeks old (Groups D & E) received 0.25ng/bird and 0.5ng.bird of AFB1 and group F is maintained as control. Experimental broilers were necropsied on day 1,3,8 and 11 of infection; controls were also necropsied on same designated days. Liver and spleen of experimental and controls were removed, weighed, homogenized and tissue samples were analyzed for protein and DNA levels. The findings clearly give a firm evidence that AFB1 brought forth morphological , physiological and biochemical changes .

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