Ecology, Environment and Conservation Paper

Vol.14, Issue 2-3, 2008; Page No.(413-475)


Ushamalini and D. Pararnesha Naik


The antimicrobial activity of some medicinal plants like Tulsi and Neem are assessed against five pathogenic microorganisms. Pathogenic organisms like Staphylococcus (lure:4s, (Gram+ye), E.Coli, Pseudomonas acruginosa, Salmonella typhymurium and Salmonella iyphii (Gram-ye) were selected for the study. Screening was performed by disc diffusion method. The zone of inhibition in Tulsi and Neem ranged from 15mm to 22mm and 18mm to 20 mm against 1.0 x 10 7 cells of each culture at 5mg concentration of herbal extract respectively. Tulsi and Neem leaf extract was most effective against E.Coli, Pseudomonas aeruginosa, and Salmonella typhii forming 22mm, 20 and 20mm and 20mm,19mm and 19mm zone of inhibition to each 5mg concentration respectively. The MIC was estimated by broth dilution technique. The biochemical alterations involved by Tulsi and Neem were inhibition and inactivation of enzyme involved in mixed acid fermentation and others. These results indicate Tulsi and Neem was a potential antimicrobial agent.

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