Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 3, 2008; Page No.(661-666)

NUTRIENT DEPLETION COUPLED WITH HIGH AUXIN CONCENTRATION TRIGGERS SERI< GENE TO INDUCE SOMATIC EMBRYO IN PLANTAGO OVATA IN VITRO

USHRI ROY, URMI ROY, ANANYA PAUL AND SARMISTHA SEN RAYCHAUDHURI

Abstract

In vitro plant regeneration of Plantago ovata was attempted through somatic embryogenesis. Shoot bud and hypocotyl sections when inoculated on MS media supplemented with 2,4-D/Kinetin formed profuse callus. After 40 days of maintenance in this medium embryogenesis was induced in these calli. The morphology of the embryos was studied following scanning electron microscope to trace the developmental pathways. SEM revealed that the protocol was a quick effective and reproducible method for plant regeneration. The callus cells maintained in the same nutrient medium with high auxin for longer duration without subculture triggered somatic embryogenesis. It is conjectured that depletion of nutrients in longterm callus culture along with high auxin content might induce expression of genes, which resulted in embryogenesis. Total RNA was extracted and RT-PCR was performed using a Somatic Embryogenesis Receptor Kinase specific primer to confirm its expression with the onset of Somatic Embryogenesis. plant growth regulators. Somatic embryogenesis

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